Knockdown of PKCδ-induced B-cell proliferation. (A) B cells or PBMCs were transfected with siRNA-universal negative control or siRNA-PRKCD (300 nM) with Amaxa electrophoration according to the Materials and Methods. After 3 days, cell lysates were prepared and subjected to the western blots (WB). (B) B cells or PBMCs were transfected with siRNA-universal negative control or siRNA-PRKCD (300 nM). The next day cells were stained with CFSE (1 μM) and stimulated with F(ab′)2 specific for anti-IgM (10 μg/mL), IL-4 (50 ng/mL), and CD40 ligand (10 μg/mL) for 5 days (for B cells) or Dynabeads T cell activator CD3/CD28 for 3 days (for PBMCs). Percent cells having undergone at least one cellular division, (numbers above the lines), assessed by CFSE dilution. Gray solid peak is unstimulated cells. Data are representative of 3 independent experiments.