CD70TG mice lack eosinophils and have increased numbers of neutrophils and monocytes. (A) Absolute numbers of neutrophils (CD11b+, Gr1+, CD115−, F4/80−), monocytes (CD11b+, CD115+, F4/80+), and eosinophils (SSChi, Siglec-F+) in bone marrow (2 femurs and 2 tibiae), spleen, and blood and of macrophages (CD11b+, F4/80+, Gr1−), mast cells (c-Kit+, FcϵR1+), neutrophils, and eosinophils in the peritoneum. (B) The presence of eosinophils was determined by analyzing expression of Siglec-F on SSChi and on CCR3+ cells (percentages are shown). (C) May-Grünwald-Giemsa staining of bone marrow smears from WT and CD70TG mice. ▴ indicates eosinophils. Images were taken using an Olympus BX51 microscope (10×/0.3 NA objective) equipped with a mounted Olympus DP70 digital camera and ACDSee 5.0 software for image acquisition. Data are mean ± SD for 3 individual mice per group. Results are representative of 3 independent experiments. *P < .05; **P < .01.
