CLL cells display significantly higher CK2 expression and activity than normal B cells. (A) Cell lysates of isolated primary CLL cells collected at diagnosis or isolated normal B cells from healthy donors were resolved by SDS-PAGE and immunoblotted with antibodies against CK2α, CK2β, or actin as loading control. Relative expression of CK2α (B) and CK2β (C) was quantified by densitometry analysis and is expressed in arbitrary units (a.u.). (D) CK2 activity was measured by an in vitro kinase assay in CLL cells (n = 8) and normal B cells (n = 6). Results shown as mean ± SEM.