Figure 6
![Figure 6. Comparison of the binding of [125I]-spectrin α18-21EF, [125I]-βABD1-4, [125I]-minispectrin, and [125I]-minispectrin-Δ13 to F-actin in the presence of protein 4.1R. The spectrin fragments and protein 4.1 were all 0.38μM. F-actin was 2.38μM in G-actin monomers. Error bars represent the range of 2 to 5 independent experiments for each protein. Neither the isolated α-spectrin EF domain nor the β-spectrin actin-binding domain binds to actin at these concentrations, whereas the assembled minispectrin binds robustly.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/14/10.1182_blood-2009-12-260612/4/zh89991058100006.jpeg?Expires=1724263203&Signature=bPnTSMrC3PhU9D5eP7qrA2PoxPJM-UNpOFiek93usWCTAJ6ou1IhPSQka8V93FpQxWoldIK-cv77f8q4UjbBveuA6DHYJWLicuetWd5kB1Ybg1uJCfoYwKkzYEbKXD8P64aOdOXXcKY~0LbifXSaxFh12TGcT3puiEC91Y5ujeIXn4PqQYKiZa~rFnKzsDQfLeIlQSYmI-AK--DifC7fXkB~sd-u8q4F2K~2PUrHktWvrt1xaeFGfZ3QRCFdnum4Er1dKAIJbltbGaYtaNC~dXxl2zlLXR7zmB9G5TzrOMxu0OHSqOnG5aTKcg7j5sAGFw3raa06u2i2rgzqiZaCDg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Comparison of the binding of [125I]-spectrin α18-21EF, [125I]-βABD1-4, [125I]-minispectrin, and [125I]-minispectrin-Δ13 to F-actin in the presence of protein 4.1R. The spectrin fragments and protein 4.1 were all 0.38μM. F-actin was 2.38μM in G-actin monomers. Error bars represent the range of 2 to 5 independent experiments for each protein. Neither the isolated α-spectrin EF domain nor the β-spectrin actin-binding domain binds to actin at these concentrations, whereas the assembled minispectrin binds robustly.
