Figure 4
![Figure 4. Protein 4.1 and minispectrin-dependent ternary complex formation. (A) Minispectrin-dependent binding of [125I]-protein 4.1 to F-actin. [125I]-Protein 4.1 (0.38μM), F-actin (2.38μM as G-actin), and increasing amounts of either minispectrin or minispectrin-Δ13 were combined, and the bound 4.1R was measured after pelleting the F-actin. (B) Protein 4.1-dependent binding of [125I]-minispectrin or [125I]-minispectrin-Δ13 to F-actin. Increasing amounts of protein 4.1R were added to the [125I]-labeled minispectrins (0.54μM) and F-actin (2.38μM), and each bound minispectrin was measured after pelleting the F-actin. Binding of the mutant minispectrin is impaired under both conditions.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/116/14/10.1182_blood-2009-12-260612/4/zh89991058100004.jpeg?Expires=1724266204&Signature=Lf3bXH8vx1b1xiHRQ3qrSoDLx3DEVMNWQX-YOmC-Yk4WOr3f-KK9U348sB7SPQzxmJZUQ9pPHncp6PIobetH~bCdXd3NS-bPuHyoYAhjw0VVc~xUTXzLWdJA9zut2VpOJaY400FaKgb2cjPkv9hXZpJlEStC75y3TYb0e3d0kr7twhm3Gm9Zc4iQybnZB2YAr-wwg9wQ-lnPNQYExfcO9PQob27ZH0NxMhGp~ywemvGulRmpIo-f7~FTiF5p~Gp8sO-EZMFPkLxXYMJRrOYc5IAe0lFbgKva2rwnnmW4lOLK1KIPz2ay-ZK1vYHxwI3gr4FGPy~KuwknQNpiP-XLHw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Protein 4.1 and minispectrin-dependent ternary complex formation. (A) Minispectrin-dependent binding of [125I]-protein 4.1 to F-actin. [125I]-Protein 4.1 (0.38μM), F-actin (2.38μM as G-actin), and increasing amounts of either minispectrin or minispectrin-Δ13 were combined, and the bound 4.1R was measured after pelleting the F-actin. (B) Protein 4.1-dependent binding of [125I]-minispectrin or [125I]-minispectrin-Δ13 to F-actin. Increasing amounts of protein 4.1R were added to the [125I]-labeled minispectrins (0.54μM) and F-actin (2.38μM), and each bound minispectrin was measured after pelleting the F-actin. Binding of the mutant minispectrin is impaired under both conditions.
