CDR3 spectratyping analysis of the TCR-δ chains in healthy adult donors and in patients after transplantation. Profiles of the CDR3 distribution of the Vδ1 (A), Vδ2 (B), and Vδ3 (C) subsets were generated from sorted CD4⁻CD8⁻ cells from CMV⁺ (n = 13) and CMV⁻ (n = 12) healthy donors. Longitudinal study of the γδ T-cell repertoire of the Vδ1, Vδ2, and Vδ3 subsets was carried out in patients during the first 12 months after SCT. (D) TCR Vγ8 chains profiles were generated from CMV⁺ (n = 19) and CMV⁻ (n = 16) healthy donors. Results are presented as mean ± SD. N indicates healthy adult; Pt, patients; and 3/12, 6/12, and 12/12 indicate the 3-, 6-, and 12-month time points after transplantation, respectively. Samples were analyzed using nonparametric Mann-Whitney test, and P values were determined. P values less than .05 were considered significant.