CMV seropositivity in healthy persons is associated with significantly higher absolute counts of Vδ2neg γδ T cells. (A) Healthy donors were divided by their CMV status into CMV⁺ (n = 23) and CMV⁻ (n = 22) groups. Samples were gated on live lymphocytes as side scatter versus forward scatter followed by gating on CD3⁺ and Vδ2pos or Vδ2neg (left) γδ T-cell subset. Absolute counts of both cell subsets were compared between the groups using nonparametric Mann-Whitney test. The horizontal bars represent the median values. P values were determined, and those less than .05 were considered significant. (B) Positive correlation between frequencies and absolute numbers of CD3⁺ Vδ2neg γδ T cells and CD8⁺CD28⁻CD57⁺ αβ T cells was detected in the CMV⁺ group.