Distinct trafficking and activation kinetics of donor T cells in GVHD. (A) Donor B6 transgenic CD5⁺ T cells (0.5 × 10⁶) were followed in BALB/c recipients (8.5 Gy) at serial time points, days (B) 2-10 after BMT. T-cell migration was monitored by the derived-constitutive extGLuc activity (blue) and T-cell activation by the NFAT-inducible CBRLuc activity (red). The overlay areas are displayed in magenta. Representative images from 1 of 5 experiments are shown. (B) Kinetics of extGLuc and CBRLuc signal intensities were quantified for PLN, gut, and spleen. Data shown represent averages ± SEM, n = 8. (C) NFAT-inducible CBRLuc activity is displayed on day 4 in vivo and on day 5 liver and gut ex vivo. Representative images from 1 of 5 experiments are shown.