Generation of chimeric mice. (A) Histograms display the transduction efficiency for LSK and lin⁻ BM cells determined by flow cytometric analysis of extGLuc expression. Positive cell numbers are shown as percentage of live (DAPI⁻) cells. Nontransduced control cells, red. Transduced cells, blue. (B) BM and T cells were harvested from chimeric B6 mice 6-8 weeks after BMT. ExtGLuc expression was determined for lin⁻ BM and CD5⁺ T cells by flow cytometry analysis. Nontransduced control cells are indicated in red, transduced cells in blue.