The intracellular region is required for PTK7 functions. We constructed a PTK7 protein deleted of the intracellular domain (PTK7.ΔKD) and transfected the construct in the Baf3 cell line. (A) Cells were sorted by flow cytometry according to PTK7 expression at the cell surface. (B) In a Western blot with anti-PTK7 antibody, amounts of PTK7 and PTK7.ΔKD proteins were comparable in Baf3-PTK7 and Baf3-PTK7.ΔKD cells. Effect of this truncated form of PTK7 was evaluated in a cell migration assay (C) and in an anthracycline-induced apoptosis assay (D). In both settings, the results obtained for the Baf3-PTK7.ΔKD cell line were comparable with Baf3-vector. Student t test was used for statistics; all P values < .05.