HRG-deficient animals fail to contain S pyogenes at the local site of infection. Tissue samples from the local site of infection from wild-type animals (A-G) and HRG-deficient animals (H-N) were hemotoxylin and eosin-stained and immunostained for inflammatory markers. Representative images are shown at 2× magnification (A,H), and the black bars define the size of the inflammatory focus that was quantified as 1375 μm in the wild-type animal and 217 μm in the HRG-deficient animal. (A,H) The black box represents the section of the inflammatory focus that is shown at 10 × magnification in all subsequent images stained for hemotoxylin and eosin (B,I), neutrophils (C,J), macrophages (D,K), S pyogenes (E,L), and the chemokines KC (F,M) and MIP-2 (G,N).