Comparison of scFv-A11 and bifunctional fusion Ab (SLK) on platelet and fibrin binding. (A) Binding ability of A11 and SLK to platelets. Platelets were coated onto 96-well plates (1 × 106/well) at 4°C overnight. Various concentrations of scFv A11 or SLK were diluted in PBS, respectively (n = 3); 13CG2 was used as a control scFv Ab. (B) Binding activity of SLK to partially digested fibrin for various time intervals. Binding of SLK to the partially digested fibrin was monitored as described in “Enzyme-linked immunosorbent assay.” The amount of SLK Ab retained on the wells was estimated by measuring the activity of bound horseradish peroxidase. Data represent the summary of 3 independent experiment results (each time point represent 3 measurements; SEM).
