Coronal sections of mouse brains showing the infarcted areas in mice given scFv A11 or saline vehicle 2 hours before carotid obstruction. (A) Mouse brains were dissected into 8 coronal planes for each mouse at 1-mm intervals. Each section was stained red with triphenyltetrazolium chloride, fixed in 10% formalin, scanned into a commercial photo-image-editing program, and the data stored on a disk. (Left) A11-treated mice. (Right) Control mice injected with saline vehicle. The unstained area of infarct was computer calculated using an automated image analysis program (Image J 1.32, National Institutes of Health). (B) Quantitation of the protective effect of A11 given 2 hours before obstruction. *Statistically significant differences by Student t test (P < .003), A11 (n = 10) versus saline control (n = 6; SEM).