Bleeding time and platelet function analysis. (A) Bleeding times were performed on Pf4/Srf WT (n = 8) and KO (n = 8) mice at the time of genotyping. *P < .01. (B-C) Platelet function analysis was performed by activation of platelets with ADP and analysis of FSC/SSC distribution (B) and analysis of GPIIb/IIIa distribution changes by staining with JON/A (C). (D) GPIIb density was confirmed by staining with anti-CD41. Data are representative of at least 3 independent experiments.