Effect of AMPK inhibition on platelet function. Human platelets were pretreated with either solvent (Sol), iodotubercidin (Iodo, 10μM) or compound C (Comp C, 10μM) and the effects of thrombin on aggregation (A), the peak increase in Ca²⁺ (B), and clot retraction (C) were assessed. The graphs summarize data from 6 independent experiments; *P < .05 and **P < .01 vs solvent (CTL)–stimulated platelets.