The Ldb1 complex and Ser2P RNA pol II are enriched at the β-globin promoter in fetal liver erythroid cells of mice homozygous for a deletion of the LCR. (A) The mouse β-globin locus is diagrammed, and the positions of TaqMan probes used for real-time PCR are indicated below and named on the graphs. Endogenous (WT) and LCR-deleted (ΔLCR) murine β-globin loci are depicted. (B) Total RNA was isolated from E14.5 fetal liver of WT or ΔLCR mice, and globin expression was analyzed by quantitative real-time RT-PCR. α-Globin was used as a positive control of RNA expression. Each value was normalized with 18S ribosomal RNA. Error bars represent the SEM for several independent RNA preparations. (C-F) Chromatin was prepared from E14.5 fetal liver of WT (+) or ΔLCR (−) mice, and then ChIP and quantitative PCR were performed with (C) Ldb1, GATA-1, LMO2, and SCL, (D) Ser2P pol II, and (E) Cdk9, or (F) DSIF and FACT components as indicated on the graphs. Necdin served as a negative control. Error bars represent the SEM among independent chromatin preparations.