Unsupervised hierarchical clustering analysis of the 139 patients with pediatric AML. (A) Two-dimensional hierarchical clustering analysis of the 139 patients was performed using 3106 probe sets that were differentially expressed in 139 patients. (B) We showed the detail of gene expression pattern focused on the FLT3, RUNX1, HOXAs, HOXBs, and HIST1s in 139 patients. Each column represents a patient and each row represents a probe set. FAB classification, biallelic CEBPA, NPM1, WT1, RUNX1, KMT2A-PTD, KMT2A rearrangements, adverse gene fusions, PRDM16 and MECOM gene expression status, and outcome of each patient are indicated. Relative expression levels normalized to the average for each probe set are indicated by color, where red and green represent high and low expression, respectively. (C) Messenger RNA expression levels of FLT3, RUNX1, PRDM16, HOXA7, HOXB3, HOXB6, HIST1H2BD, and HIST1H1C in 139 AML patients. KMT2A-R, KMT2A rearrangement.