Effect of the members of the miR-17-92 cluster on angiogenesis in human ECs. (A) Schematic illustration of the miR-17-92 cluster and sequences of the individual members. (B) HUVECs were transfected with miR precursors or a control precursor (Pre-Co) as indicated, and expression of mature miRs was detected by stem loop PCR after 24 hours. Data were normalized to RNU48; n = 3. (C-D) Inhibition of sprout formation in the spheroid assay (n = 10 spheroids/experiment; n = 5-11 experiments). *P < .05 versus Pre-Co. (D) Representative images. Scale bar represents 100 μm. (E) HUVECs were transfected with miR inhibitiors as indicated, and sprout length of spheroids was quantified (n = 10 spheroids/experiment; n = 6 experiments). *P < .05 versus MiR-Inhib-Co-2.