Active LFA-1 on mature DCs induces their adhesion to ICAM-1.(A) Mature bone marrow–derived DCs from wild-type (WT) or LFA-1^d/d mice were incubated with recombinant ICAM-1/Fc or with control immunoglobulin G (IgG), and the number of adherent cells was evaluated. Mean of n = 3 experiments (each experiment was performed in duplicate). *P < .05. (B) Cell surface markers known to be induced during maturation of DCs were expressed to a similar extent in mature wild-type and LFA-1^d/d DCs, as shown by flow cytometry (1 representative assay of 6). Surface expression of mutant compared with wild-type LFA-1 on DCs was reduced.