ULK1 depletion inhibits PML/RARA degradation. (A) HeLa cells transfected with control RNA or ULK1-targeted siRNA were retransfected with PML/RARA cDNA and treated or not with starvation media for 4 hours. Lysates were analyzed by Western blotting by use of the indicated antibodies. (B) Quantification of data from 3 independent experiments. Error bars represent SEM. *P < .05, Student t test. (C) Confirming depletion of ULK1: Lysates from control or ULK1-depleted HeLa cells were examined by Western blotting by the use of anti-ULK1 and anti-tubulin antibodies.