Sphingolipid profile of murine HSPCs. (A) Total sphingolipid content (pmol/106 cells) of Galc−/− and +/+ murine HSPCs. Each dot represents an individual sample; means are shown. (B) Intracellular content of C:16 Cer (left panel), Sph (middle panel), and S1P (right panel), in GALC or GFP LV transduced −/− HSPCs at 12 to 24 hours (h), 2 and 7 days (d) after gene transfer (n ≥ 3). *P < .05, **P < .01, ***P < .001 on 1-way ANOVA. (C) Representative images obtained after immunofluorescence staining for ceramide and for the lysosomal marker Lamp1 performed on human monocytes (U937 cells) transduced with GALC and control DeltaNGFR (DNGFR) LV 2 and 7 days after transduction. Magnification 100× in the 2 top rows and 200× in the bottom row.