MHC-I and MHC-II molecules rearrange and colocalize with ACs in IFN-α DCs upon AC uptake. CLSM analysis (central optical sections) of IFN-α DCs incubated or not with anti–LOX-1 mAb before the coculture with PHK26-PE–labeled AC. (A,C,E) After a 24-hour coculture, IFN-α DCs were fixed, made permeable, and stained with FITC-conjugated anti–MHC-I (A), anti–MHC-II (C), or with LAMP-2 (E) antibodies. Colocalization of staining is shown in merged images (yellow). In panels B and D, the up-regulation of MHC-I and MHC-II levels on unfixed IFN-α DCs was analyzed over time, after 3 and 24 hours of coculture with ACs. Scale bars correspond to 10 μm. Images were observed through a 63× oil-immersion objective lens. Data are representative of 3 independent experiments.