Hsp70-binding activity of LOX-1 in IFN-α DCs. (A) IFN-α DCs and IL-4 DCs were incubated with a neutralizing anti–LOX-1 mAb (blue lines) or isotype control anti-IgG1 mAb (red lines). After 20 minutes, Hsp70-FITC was added to the cultures and revealed by fluorescence-activated cell-sorting analysis. Black and yellow lines represent, respectively, Hsp70-treated DCs and DCs cultured alone. Results are representative of 3 independent experiments. (B) Allostimulatory capacity of IFN-α DCs cultured in the presence or absence of Hsp70. IFN-α DCs were also preincubated with anti–LOX-1 or isotype control IgG1 mAbs. The proliferation of allogeneic T cells cocultured with DCs at the indicated ratio was determined by [³H]thymidine incorporation. The results represent the mean ± SD of data obtained from 3 different experiments.