Selective LOX-1 expression in IFN-α DCs. (A) Semiquantitative PCR analysis of LOX-1 expression in IFN-α DCs and IL-4 DCs at basal levels or after LPS stimulation. Data are representative of 3 independent experiments with DCs derived from different donors. (B) LOX-1 protein expression in IFN-α DCs and IL-4 DCs cultured with or without TNF-α. All blots were probed with rat anti–human LOX-1 antiserum. LOX-1 protein appears as 3 different glycosylated forms in acrylamide gel electrophoresis. Data are representative of 4 independent experiments. (C) CLSM analysis showing the expression of LOX-1 on the surface membrane of DCs. Nuclear staining by DAPI (blue). Data are representative of 3 independent experiments. Scale bars, 10 μm.