Cytolytic activity exerted by ^p190BCR-ABL–specific BM T cells. (A) Cytotoxic activity of BMMCs after 13-day culture in the presence of ^p190BCR-ABL–derived peptides against autologous PHA blasts pulsed with ^p190BCR-ABL peptides (n = 10), autologous Ph⁺ ALL blasts (n = 3), autologous Ph⁺ ALL blasts after incubation with anti-HLA class I monoclonal antibody (W6/32, Dako; n = 2), allogeneic Ph⁺ ALL blasts (n = 4), allogeneic PHA blasts from the donor of the allogeneic Ph⁺ blasts (alloPHA*, n = 3), allogeneic Ph⁻ ALL blasts (n = 6), and allogeneic PHA blasts from the donors of the allogeneic Ph⁻ blasts (alloPHA**, n = 5). The results are represented as the number of lytic units per 10⁶ cells (LU₁₀/10⁶) and reported for each patient and as median. The LU values referring to lysis of autologous PHA blasts pulsed with ^p190BCR-ABL peptides were calculated after subtraction of background, consisting of cytotoxicity against autologous PHA blasts pulsed with irrelevant peptides. (B) Cytotoxicity profile of cultured BMMCs obtained from patient 6. The figure reports the percentage of specific lysis against autologous PHA blasts pulsed with ^p190BCR-ABL peptides (dotted line, ●) or with control peptides (dotted line, ○), autologous Ph⁺ ALL blasts (solid line, ■), allogeneic Ph⁻ ALL blasts (solid line, ▴), and allogeneic PHA blasts from the same donor of Ph⁻ blasts (solid line, ▵). The mean percentage of lysis of duplicate wells for 4 different effector-to-target (E:T) ratios is shown.