Administration of GMI-1070 after the inflammatory challenge produces the same biologic effects. Mice were treated with GMI-1070 or PBS as per protocol 2. (A) Leukocyte rolling flux (cells rolling per minute) was significantly elevated greater than 4-fold increase in SCD mice treated with GMI-1070; ***P < .001. (B) Recruitment of adherent leukocytes was severely inhibited in GMI-1070–treated SCD mice compared with PBS-treated SCD mice; **P < .01. (C) Scatter plots of the number of adherent leukocytes during the experimental period. Each dot represents data obtained from a single venule. GMI-1070 rapidly reduced the number of adherent leukocytes, compared with the PBS control group, but the number of adherent leukocytes remained almost unchanged over time after TNF-α exposure in both PBS-treated control (gray regression line) and GMI-1070–treated mice (black regression line). (D) Interactions between circulating RBCs and adherent leukocytes were abrogated (∼ 95% reduction) in SCD mice infused with GMI-1070. ***P < .001. (E) Scatter plots of the number of interactions between RBCs and adherent leukocytes during the experimental period. Each symbol represents data from a single venule. The increase in RBC-WBC interactions over time after TNF-α exposure was abrogated by the GMI-1070–treated compared with PBS-treated control mice. The gray and black regression lines represent PBS-treated and GMI-1070–treated mice, respectively.