CCR5 down-regulation in second transplanted mice. The bone marrow cells from an EGFP- and mCherry-expressing transplanted donor mouse were isolated and analyzed for CD34 (A) and EGFP and mCherry expression (B). (C-D) Bone marrow cells were directly injected into a thy/liv tissue and intravenously in irradiated recipient mice (n = 2). (C) Human CD4⁺/CD45⁺ population was identified in gated lymphocyte population in multiple tissues 14 weeks after bone marrow cell injection. (D) CCR5 expression in EGFP⁺ and EGFP⁻ population was examined in the gated mCherry⁻/CD4⁺/CD45⁺ population. Data from a representative mouse are shown.