Naive and memory T-cell differentiation of shRNA 1005-expressing T cells. (A) Naive and memory T-cell differentiation was analyzed by CD27 and CD45RA cell surface expression in gated EGFP⁺ and mCherry⁺ CD3⁺ T lymphocytes from multiple lymphoid organs. (B) A normal human peripheral blood mononuclear cell staining control is shown as a control. TCM indicates central memory T cells; TEM, effector memory T cells; and TTD, terminally differentiated cells.