Diagram of generating a lentiviral vector–transduced HPSC-transplanted hu-BLT mouse. CD34⁺ and CD34⁻ cells isolated from a human fetal liver were transduced by either shRNA (EGFP⁺) or no shRNA (mCherry⁺) vectors. The transduced cells were solidified with Matrigel and implanted under a kidney capsule with a piece of human fetal thymus. Three weeks after the implantation, the mouse was irradiated and intravenously injected with vector-transduced autologous CD34⁺ cells. FL indicates human fetal liver segment; and FT, human fetal thymus segment.