Thymectomy cannot restore CD8 tolerance in CsA-treated and NFAT1-deficient recipients. For each experiment depicted in this figure, 1 group of mice was thymectomized at the age of 4 to 5 weeks. Four weeks later, thymectomized and age-matched control mice received allogeneic BMT with 3 Gy TBI/anti-CD154. (A-B) Thymectomized WT B6 mice were treated with 3 Gy TBI/anti-CD154 mAb MR1 followed by B10.A BMT. One group of mice additionally received daily subcutaneous CsA injections for 28 days, beginning on day 0 (n = 7-10/group; 1 representative of 3 experiments is shown). (C-D) Thymectomized WT B6.129/F2 and NFAT1^−/− mice were treated with 3Gy TBI/anti-CD154 mAb and B10.A BMT (n = 7-8/group). (A,C) Incidence of chimerism is indicated for each group in different lineages. Chimerism among CD4 cells was comparable with that among CD8 cells. (B,D) Absolute levels of chimerism in mice that showed engraftment at 2 weeks is depicted over time for the granulocyte and B-cell lineages.