ODN traffics through early and late endosomes and associates with TLR9. Confocal microscopy analysis of NK cells incubated with 5 μg of TxRed-ODN (red) for 15, 30, or 45 minutes at 37°C. Cells were then stained for (A) EEA-1 (green) and KIR3DL2 (purple), (B) KIR3DL2 (green) and Rab7 (purple), or (C) TLR9 (green), as indicated. Images are single confocal sections, taken with a 60× objective, showing NK cells incubated with ODN for 15 minutes (A,C) or 30 minutes (B). Scale bar represents 2 μm. Quantitative colocalization analysis expressed as Mander coefficients of colocalization is shown. ***P < .001 was considered significant.