Genotyping assays for the tandem duplication of PLAU in QPD. Panels compare data for affected (Q) and unaffected (C) QPD family members. (A) Real-time assay findings compare the cycle thresholds (C_T) of the normal (VIC) and duplicated (FAM) allele copies for 38 affected and 54 unaffected family members, tested on the same run. C_T values of 40 indicate an undetectable genotype. NTC indicates no template controls. (B) Multiplex fragment size assays show the 207-bp and 165-bp fragments that correspond to the normal and duplicated copies of PLAU (representative subjects shown).