Figure 4
The RA1 domain of Canoe, the fly ortholog of AF6, activates the oncogenic potential of MLL. (A) Myeloid immortalization assay was performed with MLL fusion constructs indicated on the left. Each bar represents the mean ± SD of the total number of myeloid colonies per 104 plated cells. Data are derived from at least 4 cultures from 2 or more independent experiments. (B) Western blot analysis of MLL fusion proteins expressed in transiently transfected virus-producing Phoenix cells. (C) Survival curves are shown for sublethally irradiated C57BL/6 mice that received a transplant with various cell lines immortalized by MLL fusion genes.
