Thrombus formation and platelet accumulation at sites of endothelial damage in hydrodynamically injected mice after platelet transfusion. A total of 10% ferric chloride was used to injure cremaster muscle arterioles after transfusion with C57Bl/6 normal platelets. The vessels were observed for 40 minutes; fluorescence intensity was assessed every 5 minutes as a surrogate measure for platelet accumulation. (A) Time to vessel occlusion. Each symbol represents one mouse. (B) Total fluorescence accumulation, represented as the area under the curve of fluorescence intensity.