VWF multimer formation after hydrodynamic injection. Plasma samples were obtained at different time points after hydrodynamic injection and were analyzed for VWF multimer structure using 1.4% sodium dodecyl sulfate separating agarose gel electrophoresis. (A) Time course of the decreasing number of multimer bands. Data are mean ± SEM. (B) Representative multimers of plasma samples obtained at day 14. The dotted rectangle surrounds the HMW multimer bands (> 10 bands).