HDAC5 down-regulated flow-mediated KLF2 expression. (A-B) HUVECs were exposed to 1-hour flow or to static conditions after 24-hour infection with Ad-HDAC5-S/A or Ad-LacZ (control). KLF2 mRNA levels were assayed by real-time reverse-transcription (RT)–PCR (A) and protein levels were detected by KLF2 antibody (B). (C-D) HUVECs were exposed to flow or to static conditions after pretreatment with DMSO (control), W-13 (100μM), or PD 98059 (another control; 30μM) for 30 minutes. KLF2 mRNA level were assayed by RT-PCR. Vertical lines have been inserted to indicate repositioned gel lanes. *P < .05 compared with control. #P < .05 compared with that treated with flow + Ad-LacZ or DMSO. Error bars represent ±SEM