Sequence alignment of CLEC-2 family proteins. (A) Ten species of CLEC-2 were aligned using ClustalW web-based software, highlighting conservation of the DEDGYxTL motif and serines at positions 21 and 27. (B) Nine species of Dectin-1 were aligned, highlighting conservation of the DEDGYTxL motif and serines at positions 32 and 40. (C) The intracellular tails of CLEC-2, Dectin-1, and CLEC9A were aligned, highlighting conservation of the YxxL motif and partial conservation of a serine-rich region.