Cross-priming from live antigen-donor cells by DCs injected in vivo. BMDCs were cultured for 16 hours with live or apoptotic L OVA or L cells or OVA_257-264 peptide. Then DCs were sorted and injected intravenously into C57Bl/6 CD45.1 mice that were adoptively transferred the day before with CFSE-stained naive OT-I T cells. Three days later, splenocytes were restimulated with 3 μg/mL of OVA_257-264 peptide for 4 hours, and then labeled with anti-CD8, anti-CD45.2, anti-Vα2, and intracellularly with anti-IFN-γ mAb. Proliferation (A-B) and IFN-γ production (A,C) were measured by flow cytometry. Events were gated on CD8⁺Vα2⁺CD45.2⁻ cells. (B-C) Data are mean ± SEM of 3 independent experiments performed each in duplicate. n.s. indicates not significant; and n.d., not determined.