Material internalization from live donor cells by DCs in vitro. (A-C) DCs were cultured with live or apoptotic DiI-stained L cells. (A, representative of 3 experiments): dot plots of DiI capture by DCs from live cells at 4°C and 37°C after 16 hours of culture. At different time points, cells were labeled with anti-CD11c mAb (B) and annexin V (C) and analyzed by flow cytometry. Data are mean ± SEM, representative of 2 independent experiments performed in triplicates.