Tissue pathology. Prussian blue staining of kidney (A), liver (B), and spleen (C) tissue sections from (left to right) NOD control (NOD-+/+), sph^3J/sph^3J mutant, B6 control (B6-+/+), and sph^4J/sph^4J mutant mice. Note the extensive iron accumulation in kidney tubules and liver parenchyma of mutant mice. Mutant liver sections also contain numerous foci of extramedullary hematopoiesis. Bars, 100 μm.