Role of overexpressed PKCβII in VEGF-stimulated induction of PKCβ mRNA expression. (A) Western blot analysis of PKCβII and GFP expression in an endothelial cell line (EAhy926) transfected to overexpress GFP-PKCβII or GFP. (B) Effect of 100 ng/mL VEGF on PKCβ promoter-driven luciferase activity in GFP-PKCβII- and GFP-transfected endothelial cells (n = 4).