Flubendazole induces cell-cycle arrest and mitotic catastrophe. OCI-AML2 cells (A) or PPC-1 cells (B) were incubated with flubendazole or buffer control for 24 hours. Cells were then stained with PI, and the DNA content was measured by flow cytometry. A representative figure is shown. (C) PPC-1 cells were treated as in panel B and stained with anti–α-tubulin antibody and DAPI, as described in the supplemental Methods. Cells were imaged by confocal microscopy, and the number of multinucleated cells was enumerated. Data are the mean ± SD percentage of multinucleated cells from a representative experiment. *P < .001 (unpaired t test). (Inset) A representative multinucleated cell.