Release of IL-8 in response to TLR stimulation. Isolated, control and mobilized neutrophils were cultured overnight in HEPES buffer with various TLR ligands. Afterward, the supernatants were collected, and the levels of released cytokine were measured by enzyme-linked immunoabsorbent assay. Results represent data from 6 independent experiments, with cells from 6 different donors (mean ± SEM). Data were analyzed by independent 2-way analysis of variance test; *P < .05 (significant difference).