IL-8 in mobilized neutrophils. (A) mRNA was isolated from fresh control and mobilized neutrophils. Relative expression of IL-8 was assessed by quantitative RT-PCR and compared with the expression of the housekeeping gene GUS. Data were analyzed by independent 2-way analysis of variance; **P < .01 (significant difference). (B) Freshly isolated neutrophils were lysed with Triton-100–based buffer, and the levels of cell-associated IL-8 were assessed with enzyme-linked immunoabsorbent assay. Results represent the data from 3 different experiments, with cells from 3 different donors (mean ± SEM). Data were analyzed by independent 2-way analysis of variance test; *P < .05 (significant difference).