Functions of mobilized neutrophils in response to TLRs ligands. (A) Priming of NADPH oxidase activity with various TLR ligands. Neutrophils from control and GTX donors were incubated either for 30 minutes at 37°C with various TLR ligands or with TNF-α, or for 5 minutes with PAF. Afterward, fMLP-induced respiratory burst was measured by Amplex Red assay. (B) Adhesion of neutrophils to plastic surface in response to different TLR ligands. Significant difference, between control and GTX neutrophils, was observed after stimulation with all TLR ligands tested. However, this significance was absent when corrected for the basal level of adhesion, which was increased in the case of GTX neutrophils. Results represent the data from 6 different experiments (mean ± SEM). Data were analyzed by an independent 2-way analysis of variance test; *P < .05 (significant difference).