Genes and pathways deregulated in human T-ALL. (A) Heat-map image of differentially expressed genes in human T-ALL. Microarray gene expression profiling was performed on 4 human T-ALL cell lines and 34 primary T-ALL samples. Genes attaining statistical significance (P < .05, fold change ≥ 1.2) were selected and classified into 8 groups based on criteria used to classify genes in the mouse model (Table 1). The selected genes were also analyzed for significant differences in NOTCH1 mutant (MUT) versus wild-type (WT) primary T-ALL samples (20 MUTs vs 14 WTs). Genes that showed significant differences in expression levels in this comparison are indicated: *P < .05, **P < .01. (B) GSEA histograms for human T-ALL cells (cell lines, top; and primary clinical samples, bottom) representing “MYC oncogenic signature.” See the Figure 2A description for details of GSEA. The NES and the nominal P value are indicated. GSI-treated T-ALL cell lines (G), DMSO-treated T-ALL cell lines (D), wild-type primary T-ALL samples (W), and NOTCH1-mutant primary samples (M).