Figure 5
Bortezomib-resistant SUDHL16-10BR, OCI-LY10-40BR, and Raji –20BR cells exhibit partial cross-resistance to CFZ and up-regulation of proteasomal subunits. SUDHL16 and SUDHL16-10BR (A), OCI-LY10 and OCI-LY10-40BR (B), and Raji and Raji-20BR (C) cells were treated with the indicated concentration of CFZ for 36, 48, and 48 hours, respectively, after which cell death was monitored by 7-AAD staining by flow cytometry. Values represent the means for experiments performed in triplicate on 3 separate occasions plus or minus SD. (D) Logarithmically growing SUDHL16, SUDHL16-10BR, OCI-LY10, and OCI-LY10-40BR cells were harvested and proteins extracted. Western blot analysis was then performed using the indicated antibodies. Blots were stripped and reprobed with antitubulin antibodies to ensure equal loading and transfer of protein. Each lane was loaded with 20 μg of protein. Two additional experiments yielded equivalent results.

Bortezomib-resistant SUDHL16-10BR, OCI-LY10-40BR, and Raji –20BR cells exhibit partial cross-resistance to CFZ and up-regulation of proteasomal subunits. SUDHL16 and SUDHL16-10BR (A), OCI-LY10 and OCI-LY10-40BR (B), and Raji and Raji-20BR (C) cells were treated with the indicated concentration of CFZ for 36, 48, and 48 hours, respectively, after which cell death was monitored by 7-AAD staining by flow cytometry. Values represent the means for experiments performed in triplicate on 3 separate occasions plus or minus SD. (D) Logarithmically growing SUDHL16, SUDHL16-10BR, OCI-LY10, and OCI-LY10-40BR cells were harvested and proteins extracted. Western blot analysis was then performed using the indicated antibodies. Blots were stripped and reprobed with antitubulin antibodies to ensure equal loading and transfer of protein. Each lane was loaded with 20 μg of protein. Two additional experiments yielded equivalent results.

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