Signaling via podoplanin displays strong similarities to rhodocytin. (A) The level of surface expression of CHO cells stably expressing human podoplanin (hPodoCHO) was confirmed by flow cytometry using the anti-podoplanin antibody NZ-1 (infill: CHO cells; overlay: hPodoCHO cells). (B) Human washed platelets (6 × 10⁸/mL) pretreated with the indicated inhibitors were stimulated by 4 × 10⁶/mL hPodoCHO cells, and platelet aggregation was monitored by aggregometry. Data are representative of 3 independent experiments.