Immunophenotypic analysis of hematopoiesis of the mice that underwent transplantation. (A) Analysis of mixed chimerism by isotype-specific immunophenotyping. FACS profiles from peripheral blood, bone marrow, and spleen, stained with CD45.1-PE–labeled (host cells) and CD45.2–peridinin-chlorophyll-protein complex (PerCP)–labeled (donor cells) antibodies. (B) Analysis of GFP expression in the host (CD45.1) and donor compartments of peripheral blood, bone marrow, and spleen as indicated of same animals as in panel A. (C-D) Immunophenotypic analysis of bone marrow of animals that underwent transplantation. Box plots show the summarized data for immunophenotypic analyses of bone marrow from mice that received a transplant of empty vector control–PML-RARα (PRα-C; n = 7) or Pim2–PML-RARα (Pim2-PRα; n = 8). Pim2-PRα mice were leukemic at the time of analysis. Bone marrow cells were stained for the indicated surface markers as mentioned in “Flow cytometry” (nonparametric Mann-Whitney U test; B220⁺P = .045). (E) CD34 expression in the bone marrow of animals that underwent transplantation. The box plot shows the expression of CD34⁺ cells analyzed in the bone marrow of mice that underwent primary transplantation (PRα-C [n = 7] or Pim2-PRα [n = 8]; nonparametric Mann-Whitney U test; P = .03). (F) FACS profile showing forward and side scatter plots from bone marrow cells isolated from C-PRα and Pim2-PRα mice (leukemic).