Effects of Pim2 overexpression in a PML-RARα bone marrow transplantation assay. (A) Flow cytometry for GFP positivity of PML-RARα knock-in bone marrow after retroviral transduction with a GFP-containing vector (C-PRα) or a Pim2-GFP construct (Pim2-PRα) before transplantation. The dot plots show that transduction efficiency for both constructs was comparable. (B) Kaplan-Meier plot shows the probability of leukemia-free survival in animals that underwent transplantation. C-PRα indicates PRα knockin donor bone marrow was retrovirally transduced with a construct containing GFP only before transplantation; Pim2, wild-type donor bone marrow was retrovirally transduced by a construct containing Pim2 and GFP before transplantation; Pim2-PRα, PRα knock-in donor bone marrow was retrovirally transduced with a construct containing Pim2 and GFP. (C) Expression of GFP in recipient animals at the time of leukemia development (for control animals: > 7 weeks after transplantation). Data shown here are from 8 individual mice analyzed for each group for GFP fluorescence by flow cytometry. Significance was calculated by nonparametric Mann-Whitney U test. The median expression is shown by the horizontal line, the size of the box represents the borders that contain 50% of the values, and the error bars represent the upper and the lower quartiles of the values. (D) Weight distribution and photographic image of spleens of the mice that underwent transplantation at the time of leukemia development (for control animals: > 7 weeks after transplantation). Data shown here are from 8 individual mice analyzed for each group. Significance was calculated by nonparametric Mann-Whitney U test. The median expression is shown by the horizontal line, the size of the box represents the borders that contain 50% of the values, and the error bars represent the upper and the lower quartiles of the values.